New two-dimensional liquid chromatographic methods will be developed in online configuration with active focusing of the zones of transferred fractions with electrodriven principles. The possibilities of improvement of sensitivity of two-dimensional chromatography using fraction trapping columns will be tested. The effects of experimental setup, conductivity, pH, application of micellization and complex-forming additives on the efficiency of focusing of transferred zones will be evaluated. The two-dimensional separation methods combining principles of electromigration separations in the first dimension with liquid chromatography in the second dimension will be developed. New methods will be applied in the analysis of plant extracts, food samples and analysis of industrial samples of complex mixtures.

The proposed project deals with development of new instrumentation for glycoproteomic analysis. New microfluidic interfaces for on-line coupling of liquid phase separations such as capillary liquid chromatography and capillary electrophoresis will be developed. The procedure for systematic optimization of the separation conditions will be developed. The monolithic materials will be modified with nanoparticles, proteolytic and glycolytic enzymes as well as affinity ligands and integrated into the multidimensional separation systems. The coupling with mass spectrometry will be employed for identification and characterization of individual analytes. While the primary target in the proposed project will be the analysis of protein glycosylation associated with cancer and food allergy, the developed technology is expected to be of general use not only for glycoproteomic analysis, but also applicable for general characterization of complex samples.

The main subject of the research will be the role of noblewomen in the process of political and cultural communication between Central
European Hapsburg monarchy and Spanish Empire in the 16th and 17th centuries. Wedding alliances will be analyzed properly, they were a
means of bonding nobility to imperial Spanish policy and at the same time, they were a necessary requirement in the process of creating
supra-national aristocracy. That group of noble families would be connected by catholic belief and loyalty to the reigning dynasty. The author
will try to prove that noblewomen were not only passive objects of political interest but they were often given a great deal of social capital. It
enabled them to influence their husbands´ and their sons´ careers. Finally, many noblewomen had a significant position in Spanish kings´
client network due to the above mentioned factors and they played key roles in the process of implementing Spanish imperial and antireformist
policies in Central Europe.

Research and development of a new generation of functionally modified layered nanoparticles with a targeted chemical modification. The
research will result in a better incorporation of the nanoparticles into a polymer matrix, in an increased chemical stability, heat distortion
temperature, improved barrier and mechanical properties of prepared nanocomposites. The objective of chemical modification will be
mainly: (1) improved flame retardancy using modifying components based on halogen free phosphorus compounds and (2) increase
of photostability using UV absorbers.

This project is devoted to exfoliation of layered metal organophosphonates functionalized with acidic organic groups and application
of prepared nanoparticles for preparation of composites with conducting polymers. The influence of exfoliation conditions (exfoliating
agent, temperature, reaction time) on properties of nanoparticles will be studied. The properties of these nanocomposites will be
compared with properties of composites containing the same polymer and the same, but not-exfoliated layered organophosphonate.
The influence of the presence of the layered metal organophosphonate on the mechanical and thermal stability and electrical conductivity
will be studied. Prepared nanoparticles deposited onto a suitable substrate will be used for anchoring organic molecules with interesting
optical properties (luminescence, NLO-activity).

Holography is a optical technique used for recording and reconstruction of 3D objects. Holography recording is based on interference of two light beams - the beam reflected from the object surface interfere with the primary beam on the surface of a holographic film. Thus, a source of coherent radiation is an essential component of this technique. Holography is extesively employed in a number of industrial branches.

The proposed project will be divided into three main stages. The first phase of research will be aimed on preparation
of cast composite rocket propellants based on ammonium perchlorate and HTPB (hydrox terminated polybutadiene) as a binder.
The second phase of the project will be focused on the preparation of pressed composite propellants based on thermoplastics
polymers (Kraton, Estane etc.) as a binder. Burning rate of those propellants will be measured at a atmospheric pressure.
We suppose utilization of those propellants in the car and aircraft emergency systems. The third phase of the project
will be aimed on the preparation of silicone propellants with their using in the temperature range from -100 up to +100 °C.

The project will focus on the development of theoretical and practical training current and relevant issues of the occurrence of drug residues and their metabolites in the environment, including possible methods applicable to reduce (eliminate) their content in hospital wastewater, wastewater from industrial processes, respectively. seepage from landfills, etc.., for students in bachelor's, master's and doctoral programs in cooperation between the two universities: the University of Pardubice and Institute of Chemical Technology in Prague.

Thermo-physical behavior of glass-forming liquids, i.e. the crystallization and vitrification processes, are to a large extent determined
by shear viscosity. Previous studies have demonstrated that isothermal crystal growth rate corrected for thermodynamic driving force
exhibits weaker temperature dependence than viscosity (this decoupling was observed mainly for oxides and small organic molecules).
One of the main project aims is to extend this research to chalcogenide glass-formers and, based on the combined microscopic and
advanced calorimetric studies of the nucleation processes, contribute to the overall explanation of this phenomenon. The second
important project goal is to study the vitrification and structural relaxation processes and to correlate the obtained results with
the viscosity behavior of the given materials. The insight gained from this research will lead to improved control of glass formation
and microstructure development during controlled vitrification and crystallization. This is important for further development of new glassy
and glass-crystalline materials.

The project is focused on a detailed study of reversible crystallization processes as well as the structural
relaxation in phase change materials that are used for rewriteable optical or RAM data storage media.
Selected compositions of chalcogenide amorphous materials in Ge-Sb-Te and Ge-Sb-Se systems will be
prepared. Crystallization processes will be studied by using thermal analysis and various microscopy
techniques in combination with some novel methods. A systematic study of thermodynamic properties and
viscosity behavior of these compositions will be performed as an integral part of this project. The
experimental and theoretical study of structural relaxation in phase change materials is proposed. It will be
monitored by volume, enthalpy or entropy change as a function of long term annealing at selected
temperatures in the glass transition range. Such a combined approach is useful for searching new
materials with tailored properties as well as to better control the reversible crystallization process and to
predict the long term stability of amorphous phase. Both aspects are essential for further development of
phase change memory devices.