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Apart from teaching, the University of Pardubice is also renowned for its numerous scientific and research activities which contribute to an excellent national and international reputation.

Research and development (R&D) have been carried out on a broad scale, ranging from fundamental to specific applied research activities that reflect the particular needs of industry and other institutions and companies. Long-term scientific activities of the University have been carried out via institutional research projects, R&D centres, national and international grant competitions, and short-term applied research.

The University has always viewed its research, development and further creative activities as its key role. Faculties tend to fulfill the characteristics of a research-oriented institution. The University has long been in the 11th position among Czech tertiary education institutions listed in the Essential Science Indicators ISI Web of Science, based on the acceptance of authors publications. This place is mainly determined by the publications by researchers from the Faculty of Chemical Technology.

Most means to finance the research activities are gained through institutional resources. The university is currently carrying out three research plans (two at the Faculty of Chemical Technology and one at the Jan Perner Transport Faculty). Evaluation committees of the Ministry of Education, Youth and Physical Education assessed the plans in 2007, awarding the FChT grade A and the DFJP grade C.

Specific financing is based on the projects gained from different grant and programme competitions.

Four research centres have been operating at the university (three at the FChT and one at the DFJP). A major part of the specific financial means come from the Grant Agency of the Czech Republic. The degree of success at a university with a diversified spectrum of study fields is not determined merely by participation in grant competitions and by the publishing of research results, but also by varied additional creative activities, such as the publication of books for the general public in the concerned fields of interest, or by the art pieces restored by the Faculty of Restoration (FR).